土壤所施衛(wèi)明研究組使用非損傷微測(cè)技術(shù)在PCE發(fā)表文章

       近日，中國(guó)科學(xué)院南京土壤研究所施衛(wèi)明研究組在《Plant, Cell and Environment》（2009 IF 5.081）雜志發(fā)表文章，發(fā)現(xiàn)了NH₄⁺外流和GMPase的活性調(diào)節(jié)NH₄⁺抑制的根尖的生長(zhǎng)。

       根在生長(zhǎng)過(guò)程中對(duì)NH₄⁺非常敏感，過(guò)量的NH₄⁺阻礙根的生長(zhǎng)和發(fā)育，這種抑制不依賴(lài)于生長(zhǎng)素和乙烯的信號(hào)轉(zhuǎn)導(dǎo)，使用非損傷微測(cè)技術(shù)發(fā)現(xiàn)高NH₄⁺處理后伸長(zhǎng)區(qū)出現(xiàn)顯著的NH₄⁺外流，這種外流在NH₄⁺超敏感的突變體vtc1-1中更顯著，在GMPase的突變體中缺乏，因此，限制跨膜的NH₄⁺流和GMPase的功能能夠顯著降低擬南芥對(duì)NH₄⁺毒性的反應(yīng)。
       這項(xiàng)工作為植物如何避免銨鹽毒害提供了理論解釋?zhuān)瑢?duì)于理解其他非生物脅迫對(duì)根的生長(zhǎng)發(fā)育的影響有非常重要的參考價(jià)值。
       這篇文章中的NH₄⁺流速的測(cè)定在旭月（北京）科技有限公司的測(cè)試中心完成。
       目前，非損傷微測(cè)技術(shù)能夠直接測(cè)定植物的主要營(yíng)養(yǎng)物質(zhì)如K⁺、NH₄⁺和NO₃^-，這種活體測(cè)定技術(shù)是研究植物營(yíng)養(yǎng)的最佳手段。

       關(guān)鍵詞：NH₄⁺毒害，細(xì)胞伸長(zhǎng)，流速，施衛(wèi)明，非損傷微測(cè)技術(shù)，旭月

施衛(wèi)明老師簡(jiǎn)介
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Abstract：

Root growth in higher plants is sensitive to excess ammonium(NH₄⁺). Our study shows that contact of NH₄⁺ with the primary root tip is both necessary and sufficient to the
development of arrested root growth under NH₄⁺ nutrition in Arabidopsis. We show that cell elongation and not cell division is the principal target in the NH₄⁺ inhibition of primary root growth. Mutant and expression analyses using DR5:GUS revealed that the growth inhibition is furthermore independent of auxin and ethylene signalling. NH₄⁺fluxes along the primary root, measured using the Scanning Ion-selective Electrode Technique, revealed a significant stimulation of NH₄⁺ efflux at the elongation zone following treatment with elevated NH₄⁺, coincident with the inhibition of root elongation. Stimulation of NH₄⁺ efflux and inhibition of cell expansion were significantly more pronounced in the NH₄⁺-hypersensitive mutant vtc1-1, deficient in the enzyme GDP-mannose pyrophosphorylase (GMPase).We conclude that both restricted transmembrane NH₄⁺ fluxes and proper functioning of GMPase in roots are critical to minimizing the severity of the NH₄⁺ toxicity response in Arabidopsis.