茶樹(shù)應(yīng)對(duì)干旱時(shí)葉肉細(xì)胞的生理調(diào)節(jié)機(jī)制

旱害嚴(yán)重影響茶樹(shù)生長(zhǎng)發(fā)育，造成茶葉減產(chǎn)和品質(zhì)下降。揭示茶樹(shù)抗旱機(jī)理，對(duì)培育耐旱茶樹(shù)品種，應(yīng)對(duì)干旱脅迫具有重要的理論意義。

課題組以茶樹(shù)一葉為研究對(duì)象，通過(guò)PEG和復(fù)水模擬干旱和補(bǔ)水灌溉。干旱抑制茶樹(shù)葉肉細(xì)胞質(zhì)膜H⁺-ATPase活性，誘導(dǎo)H⁺內(nèi)流，介導(dǎo)膜電位去極化，激活K⁺外排，削弱了葉肉細(xì)胞對(duì)K⁺的滯留；復(fù)水激活了茶樹(shù)葉肉細(xì)胞質(zhì)膜H⁺-ATPase活性，加劇H⁺外排，超極化膜電位，抑制了K⁺外排，促進(jìn)了葉肉細(xì)胞對(duì)K⁺的滯留。因此推測(cè)葉片質(zhì)膜H⁺-ATPase可能參與調(diào)控茶樹(shù)葉片鉀穩(wěn)態(tài)對(duì)干旱和復(fù)水響應(yīng)。
 

本文第一作者，來(lái)自茶葉生物學(xué)與資源利用國(guó)家重點(diǎn)實(shí)驗(yàn)室的張顯晨博士，一直專注茶樹(shù)逆境研究，已經(jīng)利用非損傷微測(cè)技術(shù)，在茶樹(shù)氟富集、干旱脅迫、酸脅迫、鋁脅迫等方向，發(fā)表3篇SCI文章、1篇中文核心文章。

Efficient iron plaque formation on tea(Camellia sinensis) roots contributes to acidic stress tolerance. JIntegr Plant Biol. 2018, doi: 10.1111/jipb.12702.

Maintenance of mesophyll potassium andregulation of plasma membrane H⁺-ATPase are associated with physiologicalresponses of tea plants to drought and subsequent. Crop J. 2018,6(6):611-620.

Al³⁺-promoted fluorideaccumulation in tea plants (Camellia sinensis) was inhibited by an anionchannel inhibitor DIDS. J Sci Food Agric. 2016, 96(12):4224-30.

Ca²⁺ 信號(hào)在DIDS( 4，4－二異硫氰－2，2－二磺酸)抑制茶樹(shù)吸收氟的功能研究. 南京農(nóng)業(yè)大學(xué)學(xué)報(bào).2016.

​感謝張顯晨博士供稿。